G‘O‘ZA (G.HIRSUTUM L.) TURI GhFHY3/FAR1 GENLAR OILASI VAKILLARI CIS-ELEMENT HUDUDLARINI O‘RGANISH.
DOI:
https://doi.org/10.56292/SJFSU/vol31_iss5/a132Kalit so‘zlar:
G.hirsutum L., CRM, FHY3/FAR1, oqsil.Annotatsiya
O‘simliklarning o‘sish va rivojlanishi undagi mavjud genlarning murakkab tarmog‘ining shakllanishi natijasida amalaga oshadi. Bunday murakkab bog‘lanish o‘simliklarning morfologik jihatdan takomillashuviga olib keladi. Genlarning o‘zaro bog‘lanishining bir qancha yo‘llari bo‘lib, shu kabi mexanizmlardan bir bu Cis-Acting Elements (CAE) yoki cis-regulyatsiya modullarini (CRM) hisoblanadi. CRM lar funksiyasini o‘rganilishi genlar ekspressiyasini o‘rganish uchun yana bir muhim vosita hisoblanadi. O‘simliklar genomini zamonaviy texnologiyalar yordamida yuqori sifatli o‘qilishi va genomni anotatsiyalash yutuqlarga qaramay, CRM larni identifikatsiyalash, ularning funksiyalarini tushunish va gen ekspressiyasini boshqarilishdagi roli juda kam o‘rganilgan. Ushbu maqolada g‘o‘za (G.hirsutum L.) turi GhFHY3/FAR1 genlar oilasi vakillariga tegishli traskriptlarda CRM larni aniqlash bo‘yicha in-silico bioinformatik tadqiqotlar o‘tkazildi. Bu oila vakillari transkriptlarini in-silico bioinformatik tahlil qilish natijasida yorug‘likni va gibrellin, auksin, sitokinin kabi gormonlar sistemasini boshqaruvchi genlar ekspressiaysini boshqarishda ishtirok etishini ko‘rsatdi. Ushbu gormonlar tizimi o‘simliklarni o‘sishi va rivojlanishini boshqarish bilan bir qatorda ularda abiotik va biotik omillarga qarshi turish mexanizmlarini ham tartibga soladi. Mazkur tadqiqot natijalari g‘o‘za (G.hirsutum L.) turi GhFHY3/FAR1 genlar oilasi vakillariga tegishli traskriptlarda ushbu CRM larni aniqlanishi oila vakillarining abiotik va biotik omillarga qarshi turish mexanizmarini boshqarilishida ishtirok etishini ochib berdi.
Adabiyotlar
1. Wen-hai Feng, Eva Westphal, Amy Mauser, Nancy Raab-Traub, Margaret L Gulley, Pierre Busson, Shan-non C Kenney. Use of Adenovirus Vectors Expressing Epstein-Barr Virus (EBV) Immediate-Early Protein BZLF1 or BRLF1 To Treat EBV-Positive Tumors. J Virol. 2002 Nov;76(21):10951–10959. doi: 10.1128/JVI.76.21.10951-10959.2002.
2. A.P. Marand, A.L. Eveland, K. Kaufmann, N.M. Springer cis-Regulatory Elements in Plant Development, Adaptation, and Evolution Annu. Rev. Plant Biol., 74 (2023), pp. 111-137, 10.1146/annurev-arplant-070122-030236.
3. A.M. Sullivan, A.A. Arsovski, J. Lempe, K.L. Bubb, M.T. Weirauch, P.J. Sabo, R. Sandstrom, R.E. Thurman, S. Neph, A.P. Reynolds, et al. Mapping and dynamics of regulatory DNA and transcription factor networks in A. thali-ana Cell Rep., 8 (2014), pp. 2015-2030, 10.1016/j.celrep.2014.08.019.
4. D. Chen, W. Yan, L.Y. Fu, K. Kaufmann. Architecture of gene regulatory networks controlling flower devel-opment in Arabidopsis thaliana Nat. Commun., 9 (2018), pp. 4534-4613, 10.1038/s41467-018-06772-3.
5. A. Gaudinier, J. Rodriguez-Medina, L. Zhang, A. Olson, C. Liseron-Monfils, A.M. Bågman, J. Foret, S. Abbitt, M. Tang, B. Li, et al. Transcriptional regulation of nitrogen-associated metabolism and growth Nature, 563 (2018), pp. 259-264, 10.1038/s41586-018-0656-3.
6. K.A. Maher, M. Bajic, K. Kajala, M. Reynoso, G. Pauluzzi, D.A. West, K. Zumstein, M. Woodhouse, K. Bubb, M.W. Dorrity, et al. Profiling of accessible chromatin regions across multiple plant species and cell types reveals common gene regulatory principles and new control modules. Plant Cell, 30 (2018), pp. 15-36, 10.1105/tpc.17.00581.
7. Xu, X.; Mo, Q.; Cai, Z.; Jiang, Q.; Zhou, D.; Yi, J. Promoters, Key Cis-Regulatory Elements, and Their Poten-tial Applications in Regulation of Cadmium (Cd) in Rice. Int. J. Mol. Sci. 2024, 25, 13237. https://doi.org/10.3390/ijms252413237.
8. Passricha N, Saifi S, Ansari MW and Tuteja N, 2016. Prediction and validation of cis-regulatory elements in 5′ upstream regulatory regions of lectin receptor-like kinase gene family in rice. Protoplasma 1-16.
9. Liang Y, Tan ZM, Zhu L, Niu Q K, Zhou JJ, Li M and Ye D, 2013. MYB97, MYB101 and MYB120 function as male factors that control pollen tubesynergid interaction in Arabidopsis thaliana fertilization. PLoS Genet. 9(11): e1003933.
10. Lenka S and Bansal KC, 2019. Abiotic stress responsive cis-regulatory elements (CREs) in rice (Oryza sa-tiva L.) and other plants. Plant J. 13: 145-158
11. Vulavala VK, Fogelman E, Rozental L, Faigenboim A, Tanami Z, Shoseyov O and Ginzberg, 2017. Identi-fication of genes related to skin development in potato. Plant Mol.Biol. 23:1-14.
12. Ma L and Li G. FAR1-RELATED SEQUENCE (FRS) and FRS-RELATED FACTOR (FRF) Family Proteins in Arabidopsis Growth and Development. Front. Plant Sci. 9:692. (2018). doi: 10.3389/fpls.2018.00692
13. Wang, F.; Wang, X.; Zhang, Y.; Yan, J.; Ahammed, G.J.; Bu, X.; Sun, X.; Liu, Y.; Xu, T.; Qi, H.; et al. SlFHY3 and SlHY5 act compliantly to enhance cold tolerance through the integration of myo-inositol and light signaling in to-mato. New Phytol. 2022, 233, 2127–2143.
14. Chen, Y.; Deng, J.; Chen, J.; Zeng, T.; Yu, T.; Huang, Q.; Chen, P.; Liu, Q.; Jian, W.; Yang, X. Genome-wide identification and expression analysis of FAR1/FHY3 transcription factor family in tomato. Plant Physiol. J. 2021, 57, 1983–1995.
15. Li, X.; Li, Y.; Qiao, Y.; Lu, S.; Yao, K.; Wang, C.; Liao, W. Genome-Wide Identification and Expression Anal-ysis of FAR1/FHY3 Gene Family in Cucumber (Cucumis sativus L.). Agronomy 2024, 14, 50. https://doi.org/10.3390/agronomy14010050
16. Zhang, S.; Xu, F.; Zhang, Y.; Lin, J.; Song, C.; Fang, X. Fine mapping and candidate gene analysis of a novel PANICLE AND SPIKELET DEGENERATION gene in rice. Euphytica 2015, 206, 793–803.
17. Kiseleva, A.A.; Potokina, E.K.; Salina, E.A. Features of Ppd-B1 expression regulation and their impact on the flowering time of wheat near-isogenic lines. BMC Plant Biol. 2017, 17, 172.
18. Liu, Z.; An, C.; Zhao, Y.; Xiao, Y.; Bao, L.; Gong, C.; Gao, Y. Genome-Wide Identification and Characteriza-tion of the CsFHY3/FAR1 Gene Family and Expression Analysis under Biotic and Abiotic Stresses in Tea Plants (Ca-mellia sinensis). Plants 2021, 10, 570.
19. Dai, J.; Sun, J.; Peng, W.; Liao, W.; Zhou, Y.; Zhou, X.R.; Qin, Y.; Cheng, Y.; Cao, S. FAR1/FHY3 Transcrip-tion Factors Positively Regulate the Salt and Temperature Stress Responses in Eucalyptus grandis. Front. Plant Sci. 2022, 13, 883654.
20. Zeps, M.; Kondratovičs, T.; Grigžde, E.; Jansons, Ā.; Zeltiņš, P.; Samsone, I.; Matisons, R. Plantlet Anatomy of Silver Birch (Betula pendula Roth.) and Hybrid Aspen (Populus tremuloides Michx. × Populus tremula L.) Shows Intraspecific Reactions to Illumination In Vitro. Plants 2022, 11, 1097. https://doi.org/10.3390/plants11081097.
21. Wang, X.; Liu, S.; Zuo, H.; Zheng, W.; Zhang, S.; Huang, Y.; Pingcuo, G.; Ying, H.; Zhao, F.; Li, Y.; et al. Ge-nomic basis of high-altitude adaptation in Tibetan Prunus fruit trees. Curr. Biol. 2021, 31, 3848–3860.e8.
22. Du, J.; Zhang, L.; Ge, X.; Xiang, X.; Cao, D.; Yang, H.; Hu, J. Genome-Wide Identification and Characteriza-tion of the FAR1/FHY3 Family in Populus trichocarpa Torr. & Gray and Expression Analysis in Light Response. Forests 2021, 12, 1385. https://doi.org/10.3390/f12101385
23. Yuan, N.; Wang, T.; Liu, T.; Yang, Y.; Du, J. Genome-wide analysis of the FAR1/FHY3 gene family in Cot-ton. Cott. Sci. 2018, 14, 1–11.
24. Yao, H. Zheng, Y. Sun, Y. Liu, Y. Genome-Wide Identification and Expression Analysis of the FAR1-RELATED SEQUENCE (FRS) Gene Family in Grape (Vitis vinifera L.). Int. J. Mol. Sci. 2025, 26, 4675. https://doi.org/10.3390/ijms26104675
25. Huaijun Tang, De Jing 2, Cheng Liu, Xiaoqing Xie, Lei Zhang, Xunji Chen, Changyu Li. Genome-Wide Identification and Expression Analyses of the FAR1/FHY3 Gene Family Provide Insight into Inflorescence Develop-ment in Maize. Curr Issues Mol Biol. 2024 Jan 2;46(1):430–449. doi: 10.3390/cimb460107
26. Lescot, M.; Dehais, P.; Thijs, G.; Januaryal, K.; Moreau, Y.; Van de Peer, Y.; Rouze, P.; Rombauts, S. Plant-CARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter se-quences. Nucleic Acids Res. 2002, 30, 325–327.
27. Chen et al.Chen C, Wu Y, Li J, Wang X, Zeng Z, Xu J, Liu Y, Feng J, Chen H, He Y, Xia R. TBtools-II: A “one for all, all for one” bioinformatics platform for biological big-data mining. Molecular Plant. 2023a;16(11):1733–1742. doi: 10.1016/j.molp.2023.09.010.
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- Usmanov Dilshod Erkinbaevich, Аbdukarimov Sharofiddin Sayfidinovich, Sobirov Botirjon Masharif o‘g‘li, Azimov Avazxon Abbasovich, Abdug‘afforov Azamat Tojiboy o‘g‘li, Juraqulov Durbekjon Saydulla o‘g‘li, Buriev Zabardast Tojiboevich, G‘O‘ZA (G.HIRSUTUM L.) GHFHY3/FAR1 GENLAR OILASI VAKILLARINI TAVSIFLASH. , Scientific journal of the Fergana State University: № 5 (2025): FarDu ilmiy xabarlar jurnali (TABIIY FANLAR)
- Usmanov Dilshod Erkinbayevich, Аbdukarimov Sharofiddin Sayfidinovich, Sobirov Botirjon Masharif o‘g‘li, Azimov Avazxon Abbasovich, Abdug‘afforov Azamat Tojiboy o‘g‘li, Juraqulov Durbekjon Saydulla o‘g‘li, Buriev Zabardast Tojiboevich, G‘O‘ZA (GOSSYPIUM SPP) TURLARI GHFHY3/FAR1 GENLAR OILASI VAKILLARINI SOLISHTIRMA BIOINFORMATIK TAHILLAR YORDAMIDA ANIQLASH , Scientific journal of the Fergana State University: № 5 (2025): FarDu ilmiy xabarlar jurnali (TABIIY FANLAR)