sg

Chemistry

No. 5 (2020): Scientific journal of the Fergana State University

UPLC-ESI-MS METHOD APPLIED TO CHARACTERIZATION OF TWO MAJOR SAPONINS IN ALLOCHRUSA GYPSOPHILOIDES

Submitted
July 4, 2023
Published
2023-07-05

Abstract

Turkestan soap root (local name Beh, Etmak), or Allochrusa gypsophiloides is the best known endemic saponin-bearing plant of Central Asia. Ultrahigh performance liquid chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS) was applied to the analysis of the compounds of the roots of A. gypsophiloides. Through UPLC-ESI-MS studies on the plant methanol extract 2 major triterpene saponins were detected and identified as bisdesmosides of gypsogenin (A) and quillaic acid (B).

References

  1. Eisenman SW, Zaurov DE, Struwe L. Medicinal plants of Central Asia: Uzbekistan and Kyrgyzstan. New York: Springer. 2013. P. 32.
  2. Arifkhodzhaev AO. Polysaccharides of saponin–bearing plants. VII. Structural investigation of the glucogalactan from the roots of Allochrusa gypsophiloides. Chemistry of Natural Compounds.1996, 32, 523-525.
  3. Khatuntseva EA, Men'shov VM, Shashkov AS, Tsvetkov YE, Stepanenko RN, Vlasenko RY, Shults EE. Triterpenoid saponins from the roots of Acanthophyllum gypsophiloides Regel. Beilstein Journal of Organic Chemistry. 2012, 8, 763-775.
  4. Putieva ZhM, Gorovitch TT, Kondratenko ES, Abubakirov NK. Triterpene glycosides of Acanthophllum gypsophiloides. VI. Structure of acanthophylloside D. Chemistry of Natural Compounds. 1979,15, 148-151.
  5. Gevrenova R, Doytchinova I, Kołodziej B, Henry M. In-depth characterization of the GOTCAB saponins in seven cultivated Gypsophila L. species (Caryophyllaceae) by liquid chromatography coupled with quadrupole-Orbitrap mass spectrometer. Biochemical Systematics and Ecology. 2019, 83, 91-102.
  6. Gevrenova R, Bardarov K, Bouguet-Bonnet S, Voynikov Y, Balabanova V, Zheleva-Dimitrova D, Henry M. A new liquid chromatography-high resolution Orbitrap mass spectrometry-based strategy to characterize Glucuronide Oleanane-type Triterpenoid Carboxylic Acid 3, 28-O-Bidesmosides (GOTCAB) saponins.A case study of Gypsophila glomerata Pall ex M. B. (Caryophyllaceae). Journal of Pharmaceutical and Biomedical Analysis. 2018, 10, 159, 567-581.