O‘ZBEKISTONDAGI QADIMIY MAHALLIY BUG‘DOY NAVLARIDA OQSIL TARKIBIGA MA’SUL NAM-1 GENLARINI MOLEKULYAR MARKERLAR YORDAMIDA OʻRGANISH

Buronov Akmal Qalandar o‘g‘li

doi:10.56292/SJFSU/vol31_iss6/a231

Authors

Buronov Akmal Qalandar o‘g‘li

Chirchiq davlat pedagogika universiteti dotsenti,b.f.f.d

https://orcid.org/0000-0003-2185-4374

DOI:

https://doi.org/10.56292/SJFSU/vol31_iss6/a231

Keywords:

wheat, DNA, molecular markers, gene, genome, NAM A-1, NAM B-1, NAM D-1, polymerase chain reaction, protein, GPC (Grain Protein Content).

Abstract

There are various ways to improve wheat grain quality, the main one being the selection of varieties with high baking value. This article presents a PCR analysis of NAM-1 genes responsible for protein content in landrace wheat varieties of Uzbekistan using SNP markers. In the study, PCR products were successfully obtained from 20 samples of landrace wheat varieties using specific primers NAM-A-1, NAM-B-1, and NAM-D-1. This indicates the high quality of the genomic DNA used in this study.

Author Biography

Buronov Akmal Qalandar o‘g‘li, Chirchiq davlat pedagogika universiteti dotsenti,b.f.f.d

Chirchiq davlat pedagogika universiteti dotsenti,b.f.f.d

References

1. Buronov, A., Amanov, B., Muminov Kh, T. N., & Umirova, L. (2023). Polymorphism and inheritance of gliadin proteins in wheat landraces of Uzbekistan. SABRAO J. Breed. Genet, 55(3), 671-680.

2. Buronov, A. Q., Ziyayev, Z. M., Xolliyev, O. E., Fayzullayev, A. Z. (2021). Kuzgi va duvarak bug‘doy navlarinining yuqori avlod liniyalarida miqdoriy va sifat bеlgilari bo‘yicha klastеr tahlil. Academic research in educational sciences, 2(6), 1481-1488.

3. Buronov, A. Q. (2025). Collection of landrace wheat varieties bred in different ecological zones of Uzbekistan and study of valuable economic traits. Современная биология и генетика, 2(12), 78-87.

4. Buronov A.Q., Tuganova F.O., Sirojiddinov A.R (2023). Markerlarga asoslangan seleksiya texnologiyalarining donli ekinlarda qo‘llanilishi. QarDU xabarlari. 2/1(58), 132-135

5. Joshi, S. P., Gupta,V. S., Aggarwal, R. K., Ranjekar, P. K., Brar, D. S. (2000). Genetic diversity and phylogenetic relationship as revealed by inter simple sequence repeat (ISSR) polymorphism in the genus Oryza. Theor. Appl. Genet., 100, 1311 1320.

6. Jaradat A.A. Wheat Landraces; Genetik Resources for Sustinanse and Sustainability.// USDA-ARS Iowa Ave. USA. 2012. - Р. 803.

7. Juraev, D. T., Amanov, O. A., Dilmurodov, S. H. D., Meyliev, A. X., Boysunov, B. A., Aminova, D. X., & Turaeva, S. M. (2024). Bread wheat response to heat stress conditions for productivity in the Southern Regions of Uzbeki-stan.SABRAO J. Breed. Genet, 56(5), 1834-1844.

8. Lobell DB, Schlenker W, Costa-Roberts J. Climate trends and global crop production since 1980. Science 2011;333:616–20. https://doi.org/10.1126/science.1204531.

9. Semang K., Bjornstad A, Ndjiondjop M. N (2006). An Overview of Molecular Markır Methods for Plants. African Journal of Biotechnology 5 (25), 2540-2568.

10. Uauy C, Juan CB, Jorge D (2006a) The high grain protein content gene Gpc-B1 accelerates senescence and has pleiotropic effects on protein content in wheat. Journal of Experimental Botany 57, 2785–2794. doi:10.1093/jxb/erl047

11. Shiferaw B, Smale M, Braun HJ. et al. Crops that feed the world 10. Past successes and future challenges to the role played by wheat in global food security. Food Secur 2013;5:291–317. https:// doi.org/10.1007/s12571-013-0263-y.

12. Cantrell JH, Salama K. Acoustoelastic characterisation of materials. International Materials Reviews. 1991;36(1):125-145. doi:10.1179/imr.1991.36.1.125

13. Avni R, Zhao R, Pearce S, Jun Y, Uauy C, Tabbita F, Fahima T, Slade A, Dubcovsky J, Distelfeld A (2014). Functional characterization of GPC-1 genes in hexaploid wheat. Planta. 239(2), 313-324.

14. Joppa L.R,, Changheng Du, Gary E. Hart, Gary A. Hareland (1997). Mapping Gene(s) for Grain Protein in Tetraploid Wheat (Triticum turgidum L.) Using a Population of Recombinant Inbred Chromosome Lines. 37(5), 1586-1589.

15. Pearce S, Tabbita F, Cantu D, Buffalo V, Avni R, Vazquez-Gross H, Zhao R, Conley CJ, Distelfeld A, Dubcovksy J (2014). Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence. BMC Plant Biol.19(14)368. doi: 10.1186/s12870-014-0368-2. PMID: 2552423.

16. Fabien Cormier, Mickaël Throude, Catherine Ravel, Jacques Le Gouis, Magalie Leveugle, et al.. Detection of NAM-A1 Natural Variants in Bread Wheat Reveals Differences in Haplotype Distribution between a Worldwide Core Collection and European Elite Germplasm. Agronomy, 2015, 5 (2), pp.143 151.