ҲАШАРОТ ҲУЖАЙРАЛАРИНИ ПАСТ ҲАРОРАТЛИ МУҲИТДА УЗОҚ МУДДАТ САҚЛАШНИНГ ОПТИМАЛ ШАРОИТЛАРИНИ ТАНЛАШ
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Annotatsiya
Мақолада ҳашарот ҳужайралари культурасидан биотехнологик мақсадларда фойдаланиш йўллари ёритилган. Ҳужайра культуралари билан ишлаш соҳанинг муҳим объектларидан бири ҳисобланади.
Ҳужайраларни ўстириш ва сақлаш уларни ҳимоялашнинг асосий усулларидан биридир.Helicopvera zea (кўсак қурти) ва Trichoplusiani (карам тунлами) ҳашаротлари ҳужайра линияларини лаборатория шароитида кўпайтириш ва уларни узоқ муддатли паст ҳароратли (криоконсервация) муҳитда сақлашнинг оптимал усулларини аниқлашдан иборат. Тажрибалар Версенли буфер аралашмасидан фойдаланиш энг мақбул усул эканлиги исботланди. Бунда ҳужайраларнинг қайта тикланиши юқори унум билан амалга оширилди.
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