ОПТИМИЗАЦИЯ МЕТОДОВ ВЫДЕЛЕНИЯ И ОЧИСТКИ ДНК ДЛЯ МОЛЕКУЛЯРНО-ГЕНЕТИЧЕСКОГО АНАЛИЗА НЕКУЛЬТИВИРУЕМЫХ МИКРООРГАНИЗМОВ РУБЦА КОРОВ

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Abstract

This work optimizes the methods of isolation and purification of the ruminal microorganisms’ total DNA in clinically healthy cows.

Article Details

How to Cite
Л.Ильина, Г.Лаптев, Е.Йылдырым, & С.Зайцев. (2023). ОПТИМИЗАЦИЯ МЕТОДОВ ВЫДЕЛЕНИЯ И ОЧИСТКИ ДНК ДЛЯ МОЛЕКУЛЯРНО-ГЕНЕТИЧЕСКОГО АНАЛИЗА НЕКУЛЬТИВИРУЕМЫХ МИКРООРГАНИЗМОВ РУБЦА КОРОВ . Scientific Journal of the Fergana State University, (3), 4. Retrieved from https://journal.fdu.uz/index.php/sjfsu/article/view/304
Section
Chemistry

References

Van Soest P.J. Nutritional ecology of the ruminant. 2nd edn. Cornell University Press, Ithaca, New, 1994.

Hungate R.E. A roll tube method for cultivation of strict anaerobes. In: Norris J.R., Ribbons D.W. (eds). Methods in microbiology, vol. 3B. Academic Press, London and New York, p. 117–132, 1969.

Dehority B.A, Tirabasso P.A, Grifo AP. Most-probable-number procedures for enumerating ruminal bacteria, including the simultaneous estimation of total and cellulolytic numbers in one medium. Appl. Environ. Microbiol. 55:2789–2792, 1989.

Amann R.I., Ludwig W., Schleifer K.H. Phylogenetic identifi¬cation and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59:143–169, 1995.

Брюханов А.Л., Рыбак К.В., Нетрусов А.И. Молекулярная биология. М.: Изд-во МУ, 2012. -480 c.

Kocherginskaya S., Aminov, R. I. and White, B. A. Analysis of the rumen bacterial diversity under two different diet conditions using denaturing gradient gel electrophoresis, random sequencing, and statistical ecology approaches // Anaerobe. - 2001. – Vol.7. -P.119-134.

Forster R., J. Gong, and R. Teather. Group-specific 16S rRNA hybridization probes for determinative and community structure studies of Butyrivibrio fibrisolvens in the rumen // Appl. Environ. Microbiol. - 1997. – Vol.63.- P.1256-1260.

Whitehead T.R. and M.A. Cotta. Characterization and comparison of microbial populations in swine faeces and manure storage pits by 16S rDNA gene sequence analyses // Anaerobe. - 2001. – Vol.7. -P.181-187

Yu Z. and M. Morrison. Comparisons of different hypervariable regions of rrs genes for use in fingerprinting of microbial communities by PCR-denaturing gradient gel electrophoresis // Appl. Environ. Microbiol. - 2004. – Vol.70. №8.- P.4800-4806.

Flint H.J., A.M. Thomson. Deoxyribonuclease activity in isolated rumen bacteria and rumen fluid // Lett. Appl. Microbiol. - 1990. – Vol.11. -P.18-21.

Tebbe C. C. and W. Vahjen. Interference of humic acids and DNA extracted directly from soil in detection and transformation of recombinant DNA from bacteria and a yeast // Appl. Environ. Microbiol. - 1993. – Vol.59. -P.2657-2665.